SELECTED PUBLICATIONS
Tung C-C, Lobo PA, Kimlicka L, Van Petegem F (2010) The ryanodine receptor N-terminal disease hot spot forms a cytoplasmic vestibule. Nature 468, 585-588.
Sarhan M, Van Petegem F*, Ahern CA* (2012) Crystallographic basis for calcium regulation of sodium channels. Proc. Nat. Acad. Sci. USA 109, 3558-3563. * Co-corresponding authors
Kimlicka L, Lau K, Tung CC, Van Petegem F (2013) Disease mutations in the Ryanodine Receptor N-terminal region couple to a mobile intersubunit interface. Nature Commun. 4:1506.
Lau K, Van Petegem F (2014) Crystal Structures of the Ryanodine Receptor SPRY2 domain: a clamp domain targeted by destabilizing disease mutations. Nature Commun. 5:5397
Yuchi Z, Wong King Yuen S, Lau K, Underhill AQ, Cornea R, Fessenden J, Van Petegem F (2015) Crystal structures of Ryanodine Receptor SPRY1 and tandem-repeat domains reveal a critical FKBP12 binding determinant. Nature Commun. 6,7947.
Das S, Gilchrist J, Bosmans F, Van Petegem F (2016) Binary architecture of the Nav1.2-β2 signaling complex. eLife 5: e10960
Wong King Yuen SM, Campiglio M, Tung CC, Flucher BE, Van Petegem F (2017) Structural insights into binding of STAC proteins to voltage-gated calcium channels. Proc. Natl. Acad. Sci. USA.114:E9520-E9528.
Wang K, Holt C, Lu J, Brohus M, Larsen KT, Overgaard MT, Wimmer R, Van Petegem F (2018) Arrhythmia mutations in Calmodulin cause conformational changes that affect interactions with the cardiac voltage-gated calcium channel. Proc. Natl. Acad. Sci. USA. 115:E10556-E10565.
Haji-Ghassemi O, Yuchi Z, Van Petegem F (2019) The Cardiac Ryanodine Receptor Phosphorylation Hot Spot Embraces PKA in a Phosphorylation-Dependent Manner. Molecular Cell. 75, 1-14.
Gardill B, Tung C-C, Van Petegem F (2019) Crystal structures of Ca2+-calmodulin bound to NaV C-terminal regions suggest role for EF-hand domain in binding and inactivation. Proc. Natl. Acad. Sci. USA. 116,10763-10772.
Ma R, Haji-Ghassemi O, Ma D, Jiang H, Lin L, Yao L, Samurkas A, Li Y, Wang Y, Cao P, Wu S, Zhang Y, Murayama T, Moussian B, Van Petegem F*, Yuchi Z*. (2020) Structural basis for diamide modulation of ryanodine receptor. Nature Chem Biol. doi: 10.1038/s41589-020-0627-5. *co-corresponding authors.
Woll K, Haji-Ghassemi O, Van Petegem F (2021) Pathological conformations of disease mutant Ryanodine Receptors revealed by cryo-EM. Nature Comm. 12:807
Yang ZF, Panwar P, McFarlane C, Wietske ET, Campiglio M, Van Petegem F (2022) Structures of the junctophilin/voltage-gated calcium channel interface reveal hot spot for cardiomyopathy mutations. Proc. Natl. Acad. Sci. USA. 119, e2120416119
Haji-Ghassemi O, Chen YS, Woll K, Gurrola GB, Valdivia CR, Cai W, Li S, Valdivia HH, Van Petegem F (2023) Cryo-EM analysis of scorpion toxin binding to Ryanodine Receptors reveals subconductance that is abolished by PKA phosphorylation. Science Advances. 9:eadf4936
All publications by Filip Van Petegem
2024
119. Pfeil-Gardiner O, Dias Rosa HV, Riedel D, YChen YS, Lorks D, Kukelhan P, Linck M, Muller H, Van Petegem F, Murphy BJ. (2024) Localising elements in single-particle reconstructions by REEL-EM: Reconstructed Electron Energy-Loss - Elemental Mapping. BioRXiv.
https://www.biorxiv.org/content/10.1101/2024.01.18.575858v1
118. Joseph TT, Bu W, Haji-Ghassemi O, Chen YS, Woll K, Allen PD, Brannigan G, Van Petegem F, Eckenhoff RG (2024) Propofol directly binds and inhibits skeletal muscle ryanodine receptor 1 (RyR1). bioRXiv. doi: 10.1101/2024.01.10.575040
117. Loutet SA, Cheung S, Zaytsoff S, Hofacre C, Jones MK, Van Petegem F, Abnousi H (2024) Research note: Clostridium perfringens NetB and CnaA neutralizing nanobodies in feed reduce the incidence of poultry necrotic enteritis. Poult. Sci. 103:103578
2023
116. Yoo R, Haji-Ghassemi O, Xu J, McFarlane C, Van Petegem F (2023) Crystallographic, kinetic, and calorimetric investigation of PKA interactions with L-type calcium channels and Rad GTPase. BioRXiv. https://doi.org/10.1101/2023.10.24.563811
115. Seflova J, Schwarz JA, Svensson B, Blackwel DJ, Smith AN, Phillips TA, Nikolaienko R, Bovo E, Thrope MP, Rebbeck RT, Zima AV, Thomas DD, Van Petegem F, Knollmann BJ, Johsnton JN, Robia SL, Cornea RL (2023) RyR2 Binding of an Antiarrhythmic Cyclic Depsipeptide Mapped Using Confocal Fluorescence Lifetime Detection of FRET. ACS Chemical biology. 18,2290-2299
114. Haji-Ghassemi O, Chen YS, Woll K, Gurrola GB, Valdivia CR, Cai W, Li S, Valdivia HH, Van
Petegem F (2023) Cryo-EM analysis of scorpion toxin binding to Ryanodine Receptors reveals subconductance that is abolished by PKA phosphorylation. Science Advances. 9:eadf4936
113. Cholak S, Saville JW, Zhu X, Berezuk AM, Tuttle KS, Haji-Ghassemi O, Alvarado FJ, Van Petegem
F*, Subramaniam S* (2023) Allosteric modulation of ryanodine receptor RyR1 by nucleotide derivatives. Structure. In press. (doi: 10.1016/j.str.2023.04.009)
112. Kwan JZJ, Nguyen T, Uzozie A, Budzynski MA, Cui J, Lee JMC, Van Petegem F, Lange PF, Teves
SS (2023) RNA Polymerase II transcription independent of TBP. eLife. 12:e83810.
111. O’Connor TN, van den Bersselaar LR, Chen YS, Nicolau S, Simon B, Huseth A, Todd JJ, Van
Petegem F, Sarkozy A, Goldberg MF, Voermans NC, Dirksen RT (2023) RyR-1-Related Diseases International Workshop: From Mechanisms to Treatments. J. Neuromusc. Dis. 10,135-154
2022
110. Pesch YY, Dang V, Fairchild MJ, Islam F, Camp D, Kaur P, Smendziuk CM, Messenberg A, Carr R,
McFarlane CR, Musso PY, Van Petegem F, Tanentzapf G (2022). Gap junctions mediate discrete regulatory steps during fly spermatogenesis. PloS Genet. 18(9):e1010417
109. Rayani K, Hantz ER, Haji-Ghassemi O, Li AY, Spuches AM, Van Petegem F, Solaro RJ, Lindert S,
Tibbits GF (2022) The effect of Mg2+ and Ca2+ binding to cardiac troponin C in hypertrophic cardiomyopathy associated TNNC1 variants. FEBS J. In press.
108. Willegems K, Eldstrom J, Kyriakis E, Ataei F, Sahakyan H, Dou Y, Russo S, Van Petegem F*,
Fedida F* (2022) Structural and electrophysiological basis for the modulation of KCNQ1 channel currents by ML277. Nat Commun 13:3760 *co-corresponding authors
107. Qin J, Zhang J, Lin L, Haji-Ghassemi O, Lin Z, Woycechowsky KJ, Van Petegem F, Zhang Y, Yuchi
Z (2022) Structures of PKA-phospholamban complexes reveal a mechanism of familial dilated cardiomyopathy. Elife. 11:e75346.
106. Yang ZF, Panwar P, McFarlane C, Wietske ET, Campiglio M, Van Petegem F (2022) Structures of
the junctophilin/voltage-gated calcium channel interface reveal hot spot for cardiomyopathy mutations. Proc. Natl. Acad. Sci. USA. 119, e2120416119
105. Arslanova A, Shaffaattalab S, ye K, Asghari P, Lin L, Kim B, Roston TM, Hove-Madsen L, Van
Petegem F, Sanatani S, Moore E, Lynn F, Sondergaard M, Luo Y, Chen SRW, Tibbits GF (2022) Using hiPSC-CMs to Examine Mechanisms of Catecholaminergic Polymorphic Ventricular Tachycardia. Curr Protoc. 1:e320
104. Rossi D, Lorenzini S, Pierantozzi E, Van Petegem F, Amadsun DO, Sorrentino V (2022) Multiple
regions of junctin drive interaction with calsequestrin-1 and localization at triads in skeletal muscle. J. Cell Sci. 135:jcs259185.
103. Zhang J, Singh DP, Ko CY, Nikolaienko R, Wong King Yuen SM, Schwarz JA, Treinen LM, Tung
CC, Rozman K, Svensson B, Aldrich CC, Zima AV, Thomas DD, Bers DM, Launikonis BS, Van Petegem F, Cornea RL (2022) Cardiac ryanodine receptor N-terminal region biosensors identify novel inhibitors via FRET-based high-throughput screening. J.Biol.Chem. 15:101412
102. Grondin J, Dejean G, Van Petegem F, Brumer H (2022) Cell-surface xyloglucan recognition and
hydrolysis by the human gut commensal Bacteroides uniformis. Appl. Environ. Microbiol. 88:e0156621
2021
101. Scortecci JF, Molday LL, Curtis SB, Garces FA, Panwar P, Van Petegem F, Molday RS (2021).
Cryo-EM structures of the ABCA4 importer reveal mechanisms underlying substrate binding and Stargardt disease. Nature Comm. 12:5902
100. Xue J, Purushotham P, Acheson JF, Ho R, Zimmer J, McFarlane C, Van Petegem F, Martone PT,
Samuels AL (2022) Functional characterization of a cellulose synthase, CtCESA1, from the marine red alga Calliarthon tuberculosum (Corallinales). J. Exp. Bot. 73:680-695
99. Woll K, Van Petegem F (2022) Calcium release channels: structure and function of IP3 Receptors and Ryanodine Receptors. Physiol.Rev. 102:209-268.
98. Arslanova A, Shaffatalab S, Ye K, Asghari P, Lin L, Kim B, Roston TM, Hove-Madsen L, Van
Petegem F, Sanatani S, Moore E, Lynn F, Sondergaard M, Luo Y, Chen SRW, Tibbits GF (2021) Curr. Protoc. 1:e320
97. Rufenach B, Van Petegem F (2021) Structure and function of STAC proteins: Calcium channel modulators and critical components of muscle excitation-contraction coupling. J. Biol. Chem. In Press.
96. Van den Bersselaar LR, Greven T, Bulger T, Voermans NC, Van Petegem F, Shiemann AH, Parker R, Burling SM, Jungbluth H, Stowell KM, Kamsteeg EJ, Snoeck MMJ (2021) RYR1 variant c.38T>G, p.Leu13Arg causes hypersensitivity of the ryanodine receptor-1 and is pathogenic for malignant hyperthermia. Br.J.Anaesth. S0007-0912(21)00299-3
95. Jain N, Tamura K, Dejean G, Van Petegem F, Brumer H (2021) Orthogonal Active-Site Labels for Mixed-Linkage endo-β-Glucanases. ACS Chem Biol. In Press. Doi: 10.1021/acschembio.1c00063.
94. Scala M, Stephanie E, Sultan T, De Waele J, Panciroli M, Maroofian R, Striano P, Van Petegem F, Houlden H, Bosmans F (2021) Homozygous SCN1B variants causing early infantile epileptic encephalopathy 52 affect voltage-gated sodium channel function. Epilepsia. 62, e82-e87.
93. Safabaksh S, Panwar P, Barichello S, Sangha SS, Hanson PJ, Van Petegem F, Laksman Z (2021) The role of phosphorylation in atrial fibrillation: a focus on mass spectrometry approaches. Q Cardiovasc. Res. Doi: 10.1093/cvr/cvab095. Online ahead of print.
92. Tamura K, Dejean G, Van Petegem F, Brumer H (2021) Distinct protein architectures mediate species-specific beta-glucan binding and metabolism in the human gut microbiota. J. Biol. Chem. Doi: 10.1016/j.jbc.2021.100415
91. Rayani K, Seffernick J, Li AY, David JP, Spuches AM, Van Petegem F, Solaro RJ, Lindert S, Tibbits GF (2021) Binding of calcium and magnesium to human cardiac Troponin C. J. Biol. Chem doi 10.1016/j.jbc.2021.100350
90. Woll K, Haji-Ghassemi O, Van Petegem F (2021) Pathological conformations of disease mutant Ryanodine Receptors revealed by cryo-EM. Nature Comm. 12:807
2020
89. Genovese I, Giamogante F, Barazzuol L, Battista T, Fiorillo A, Vicario M, D’Alessandro G, Cipriani R, Limatola C, Rossi D, Sorrentino V, Poser E, Mosca L, Squitieri F, Perluigi M, Arena A, Van Petegem F, Tito C, Fazi F, Giorgi C, Cali T, Ilari A, Colotti G (2020) Sorcin is an early marker of neurodegeneration, Ca2+ dysregulation and Endoplasmic Reticulum Stress associated to neurodegenerative diseases. Cell Death and Disease. 11:861
88. Llongueras JP, Das S, De Waele J, Capulzini L, Sorgente A, Van Petegem F*, Bosmans F* (2020) Biophysical Investigation of Sodium Channel Interaction with β-Subunit Variants Associated with Arrhythmias. Bioelectricity. In Press. *co-corresponding authors
87. Ibarra Moreno CA, Kraeva N, Zvaritch E, Figueroa L, Rios E, Biesecker L, Van Petegem F, Hopkins PM, Riazi S (2020) A multi-dimensional analysis of genotype-phenotype discordance in malignant hyperthermia susceptibility. Br J Anaesth. 2020 Aug 26:S0007-0912(20)30633-4. doi: 10.1016/j.bja.2020.07.042.
86. Chamberlain TC, Cheung ST, Yoon JSJ, Ming-Lum A, Gardill BR, Shakibakho S, Dzananovic E, Ban F, Samiea A, Jawanda K, Priatel J, Krystal G, Ong CJ, Cherkasov A, Andersen RJ, McKenna SA, Van Petegem F, Mui AL (2020) Interleukin-10 and Small Molecule SHIP1 Allosteric Regulators Trigger Anti-inflammatory Effects through SHIP1/STAT3 Complexes. iScience. 23(8):101433.
85. Ma R, Haji-Ghassemi O, Ma D, Jiang H, Lin L, Yao L, Samurkas A, Li Y, Wang Y, Cao P, Wu S, Zhang Y, Murayama T, Moussian B, Van Petegem F*, Yuchi Z*. (2020) Structural basis for diamide modulation of ryanodine receptor. Nat Chem Biol. doi: 10.1038/s41589-020-0627-5. *co-corresponding authors.
84. Rufenach B, Christy D, Flucher BE, Bui JM, Gsponer J, Campiglio M, Van Petegem F (2020) Multiple Sequence Variants in STAC3 Affect Interactions with Ca V 1.1 and Excitation-Contraction Coupling. Structure. 28(8):922-932.
83. Holt C, Hamborg L, Lau K, Brohus M, Sørensen AB, Larsen KT, Sommer C, Van Petegem F*, Overgaard MT*, Wimmer R*. (2020) The arrhythmogenic N53I variant subtly changes the structure and dynamics in the calmodulin N-terminal domain, altering its interaction with the cardiac ryanodine receptor. J. Biol. Chem. 295(22):7620-7634 * co-corresponding authors
82. Dejean G, Tamura K, Cabrera A, Jain N, Pudio N, Pereira G, Viborg A, Van Petegem F, Martens E, Brumer H (2020) Synergy between cell-surface glycosidases and glycan-binding proteins dictates the utilization of specific beta(1,3)-glucans by human gut Bacteroides. mBio. 11:e00095-20
81. Stringer RN, Jurkovicova-Tarabova B, Huang S, Haji-Ghassemi O, Idoux R, Liashenko A, Souza IA, Rzhepetskyy Y, Lacinova L, Van Petegem F, Zamponi GW, Pamphlett R, Weiss N (2020) A rare CACNA1H variant associated with amyotrophic lateral sclerosis causes complete loss of Cav3.2 T-type channel activity. Mol. Brain. In press.
80. Wang K, Brohus M, Holt C, Overgaard MT, Wimmer R, Van Petegem F (2020) Arrhythmia mutations in calmodulin can disrupt cooperativity of Ca2+ binding and cause misfolding. J. Physiol. In Press doi: 10.1113/JP279307.
79.Tung M, Van Petegem F, Lauson S, Collier A, Hodkinson K, Fernandez B, Connors S, Leather R, Sanatani S, Arbour L (2020) Cardiac arrest in a mother and daughter and the identification of a novel RYR2 variant predisposing to low penetrant Catecholaminergic Polymorphic Ventricular Tachycardia in a 4 generation Canadian family. Mol. Genet. Genomic Med. e1151. doi: 10.1002/mgg3.1151
78. Gardill B, Huang J, Tu L, Van Petegem F, Oxenoid K, Thomson C (2020) Nanodisc technology f acilitates identification of monoclonal antibodies targeting multi-pass membrane proteins. Sci. Rep. 10(1):1130.
77. Asghari P, Scriven DR, Ng M, Panwar P, Chou KC, Van Petegem F, Moore ED (2020) Cardiac ryanodine receptor distribution is dynamic and changed by auxiliary proteins and post- translational modification. Elife. 9. pii: e51602.
76. Round P, Das S, Wu TS, Wahala K, Van Petegem F, Hammond GL (2020) Molecular interactions between sex hormone-binding globulin and non-steroidal ligands that enhance androgen action. J. Biol. Chem. 295, 1202-1211
2019
75. Ng L, Zhuang M, Van Petegem F, Li YX, Accili EA (2019) Binding and structural asymmetry governs ligand sensitivity in a cyclic nucleotide-gated ion channel. J.Gen.Physiol. 151,1190-1212
74. Mellor GJ, Panwar P, Lee AK, Steinberg C, Hathaway J, Bartels C, Christian S, Balaji S, Roberts JD, Simpson CS, Boczek NJ, Tester DJ, Radbill AE, Mok N-S, Hamilton R, Kaufman E, Eugenio PL, Weiss R, January C, McDaniel GM, Leather RA, Erickson C, Falik S, Behr ER, Wilde AAM, Sanatani S, Ackerman MJ, Van Petegem F, Krahn AD, Laksman Z (2019) Type 8 Long QT Syndrome: Pathogenic Variants in CACNA1C-Encoded Cav1.2 Cluster in STAC Protein Binding Site. Europace. In Press. CA
73. Gardill B, Tung C-C, Van Petegem F (2019) Crystal structures of Ca2+-calmodulin bound to NaV C- terminal regions suggest role for EF-hand domain in binding and inactivation. Proc. Natl. Acad. Sci. USA. 116,10763-10772.
72. Haji-Ghassemi O, Yuchi Z, Van Petegem F (2019) The Cardiac Ryanodine Receptor Phosphorylation Hot Spot Embraces PKA in a Phosphorylation-Dependent Manner. Molecular Cell. 75,1-14
71. Tamura K, Foley MH, Gardill BR, Dejean G, Schnizlein M, Bahr CME, Louise Creagh A, van Petegem F, Koropatkin NM, Brumer H (2019) Surface glycan-binding proteins are essential for cereal beta-glucan utilization by the human gut symbiont Bacteroides ovatus. Cell Mol. Life Sci. 76,4319-4340.
70. Alvarado FJ, Bos JM, Yuchi Z, Valdivia CR, Hernandez JJ, Zhao YT, Henderlong DS, Chen Y, Booher TR, Marcou C, Van Petegem F, Ackerman MJ, Valdivia HH (2019) Cardiac Hypertrophy and Arrhythmia in Mice Harboring a Novel RyR2 Missense Variant Associated with Hypertrophic Cardiomyopathy. J. Clin. Invest. Insight 5.pii: 126544. DOI 10.1172/jci.insight.126544.
69. Shafaattalab S, Li AY, Lin E, Stevens CM, Dewar L, Lynn F, Sanatani S, Laksman Z, Van Petegem F, Madsen LH, Tieleman P, Davis J, Tibbits GF (2019) Arrhythmogenic cardiac thin filament mutations as a potential cause of sudden cardiac death in infants: post-mortem in vitro analyses to determine causality. Proc. Natl. Acad. Sci. USA. 116(14):6969-6974.
68. Haji-Ghassemi O, Muller-Loennnies S, Brooks CL, MacKenzie CR, Caveney N, Van Petegem F, Brade L, Kosma P, Brade H, Evans SV (2019) Subtle changes in the combining site of the Chlamydiaceae-specific mAb S25-23 increase antibody-carbohydrate binding affinity by an order of magnitude. Biochemistry. 58, 714-726.
2018
67. Brohus M, Sondergaard MT, Chen SR, Van Petegem F, Overgaard MT (2018) Ca2+ dependent calmodulin binding to cardiac ryanodine receptor (RyR2) calmodulin-binding domains. Biochemical J. 476,193-209.
66. Roston T*, Haji-Ghassemi O*, LaPage MJ, Batra A, Bar-Cohen Y, Anderson C, Lau YR, Maginot K, Gebauer RA, Etheridge SP, Potts JE, Van Petegem F, Sanatani S (2018) Catecholaminergic polymorphic ventricular tachycardia patients with multiple genetic variants in the PACES CPVT Registry. Plos One. 3,e0205925.
65. Wang K, Holt C, Lu J, Brohus M, Larsen KT, Overgaard MT, Wimmer R, Van Petegem F (2018) Arrhythmia mutations in Calmodulin cause conformational changes that affect interactions with the cardiac voltage-gated calcium channel. Proc. Natl. Acad. Sci. USA. 115:E10556-E10565.
64. Pancaroglu R, Van Petegem F (2018) Calcium channelopathies: structural and functional insights into disorders of the muscle excitation-contraction complex. Ann. Rev. Genet. 52:373-396. doi: 10.1146/annurev-genet-120417-031311
63. Gardill B, Rivera-Acevedo RE, Tung C-C, Okon M, McIntosh LP, Van Petegem F (2018). The voltage-gated sodium channel EF-hands forms an interaction with the III-IV linker that is disturbed by disease-causing mutations. Scientific Rep. 8(1):4483.
62. Campiglio M, Coste de Bagneaux P, Ortner NJ, Tuluc P, Van Petegem F, Flucher BE (2018) STAC proteins associate to the IQ domain of CaV1.2 and inhibit calcium-dependent inactivation. Proc. Natl. Acad. Sci. USA 115, 1376-1381
2017
61. Wong King Yuen SM, Campiglio M, Tung CC, Flucher BE, Van Petegem F (2017) Structural insights into binding of STAC proteins to voltage-gated calcium channels. Proc. Natl. Acad. Sci. USA.114:E9520-E9528.
60. Liu Y, Wei J, Wong King Yuen SM, Sun B, Tang Y, Wang R, Van Petegem F, Chen SRW (2017) CPVT-associated cardiac ryanodine receptor mutation G357S with reduced penetrance impairs Ca2+ release termination and diminishes protein expression. PLoS One 12,e0184177
59. Roston T, Yuchi Z, Kannankeril PJ, Hathaway J, Vinocur JM, Etheridge SP, Potts JE, Maginot KR, Salerno JC, Cohen M, Hamilton RM, Pflaumer A, Mohammed S, Kimlicka L, Kanter RJ, LaPage MJ, Collins KK, Gebauer RA, Temple JD, Batra AS, Erickson C, Miszczak-Knecht M, Kubus P, Bar-Cohen Y, Kantoch M, Thomas VC, Hessling G, Anderson C, Young ML, Choi SHJ, Cabrera Ortega M, Lau YR, Johnsrude CL, Fournier A, Van Petegem F, Sanatani S (2016). The Clinical and Genetic Spectrum of Catecholaminergic Polymorphic Ventricular Tachycardia: Identifying Predictors of Arrhythmia in an International Multicenter Registry. Europace. 20(3):541-547.
58. Roston T, Van Petegem F,Sanatani S (2017) Catecholaminergic Polymorphic Ventricular Tachycardia: a model for Genotype-Specific Therapy. Curr. Opinion in Cardiol. 32(1):78-85.
57. Sondergaard MT, Liu Y, Larsen KT, Nani A, Tian X, Holt C, Wang R, Wimmer R, Van Petegem F, Fill M, Chen SR, Overgaard MT (2017) The Arrhythmogenic Calmodulin p.Phe142Leu Mutation Impairs C-domain Ca2+-binding but not Calmodulin-dependent Inhibition of the Cardiac Ryanodine Receptor. J. Biol. Chem. 292(4):1385-1395.
56. McGregor N, Yin V, Ching-Chieh T, Van Petegem F,Brumer H (2017) Crystallographic insight into the evolutionary origins of xyloglucan endo-transglycosylases and endo-hydrolases. Plant.J. 89(4):651-670.
55. Roston T, Guo W, Krahn AD, Wang R, Van Petegem F, Sanatani S, Chen SR, Lehman A (2017) A novel RyR2 Loss-of-Function Mutation (I4855M) is Associated with Left Ventricular Non-compaction and Atypical Catecholaminergic Polymorphic Ventricular Tachycardia. J. Electrocardiol. 50,227-233.
2016
54. Ng L, Putrenko I, Baronas V, Van Petegem F, Accili E (2016) Cyclic purine and pyrimidine nucleotides bind to the HCN2 ion channel and variably promote C-terminal domain interactions and opening. Structure. 24, 1629-1642.
53. Stevens C., Rayani K, Genge C, Singh G, Liang B, Roller J, Li C, Li A, Tieleman P, Van Petegem F, Tibbits G (2016) Functional Characterization of Cardiac and Slow Skeletal Troponin C Paralogs in Zebrafish by MD Simulation and Isothermal Titration Calorimetry. Biophysical J. 111,38-49
52. Yuchi Z, Van Petegem F (2016) Ryanodine Receptors under the Magnifying Lens: insights and limitations of cryo-electron microscopy and X-ray crystallography studies. Cell Calcium. 59, 209-227.
51. Alzayady KJ, Wang L, Chadrasekhar R, Wagner II LE, Van Petegem F, Yule D (2016) Defining the Stoichiometry of Inositol 1,4,5-Trisphosphate Binding Required to Initiate Ca2+ Release. Science Signaling. 9(422):ra35.
50. Vashchenko G, Das S, Moon KM, Rogalski JC, Taves MD, Soma KK, Van Petegem F, Foster LJ, Hammond GL (2016) Identification of avian corticosteroid-binding globulin (SerpinA6) reveals the molecular basis of evolutionary adaptations in SerpinA6 structure and function as a steroid-binding protein. J. Biol. Chem. 291,11300-11312
49. Das S, Gilchrist J, Bosmans F, Van Petegem F (2016) Binary architecture of the Nav1.2-β2 signaling complex. eLife 5: e10960
2015
48. Xiao Z, Guo W, Wong King Yuen S, Wang R, Zhang L, Van Petegem F, Chen SR (2015) The H29D mutation does not enhance cytosolic Ca2+ activation of the cardiac Ryanodine Receptor. Plos One 10:e0139058.
47. Brath U, Swamy SI, Veiga AX, Tung CC, Van Petegem F, Erdelyi M (2015) Paramagnetic Ligand Tagging to Identify Protein Binding Sites. J. Am. Chem. Soc. 137,11391-11398.
46. Yuchi Z, Wong King Yuen S, Lau K, Underhill AQ, Cornea R, Fessenden J, Van Petegem F (2015) Crystal structures of Ryanodine Receptor SPRY1 and tandem-repeat domains reveal a critical FKBP12 binding determinant. Nature Comm. 6,7947.
45. Liu Y, Xiao Z, Wang R, Guo W, Zhang JZ, Mi T, Wang Y, Jones PP, Van Petegem F, Chen SRW (2015) Roles of the NH2-terminal Domains of Cardiac Ryanodine Receptor in Ca2+ Release Activation and Termination. J. Biol. Chem. 290, 7736-7743.
44. Van Petegem F (2015). Ryanodine Receptors: Allosteric Ion Channel Giants. J. Mol. Biol. 427,31-53
2014
43. Lau K, Van Petegem F (2014) Crystal Structures of the Ryanodine Receptor SPRY2 domain: a clamp domain targeted by destabilizing disease mutations. Nature Comm. 5:5397.
42. Trane AE, Pavlov D, Sharma A, Saqib U, Lau K, Van Petegem F, Minshall RD, Roman LJ, Bernatchez PN (2014) Deciphering the binding of caveolin-1 to client protein endothelial nitric oxide synthase (eNOS): scaffolding sub-domain identification, interaction modeling, and biological significance. J. Biol. Chem. 289,13273-13283.
41. Lau K, Chan M, Van Petegem F (2014) lobe-specific calmodulin binding to different ryanodine receptor isoforms. Biochemistry 53,932-946.
40. Brath U, Lau K, Van Petegem F, Erdelyi M (2014) Mapping the sevoflurane-binding sites of calmodulin. Pharmacology Res. Perspectives 2:5 (DOI: 10.1002/prp2.25)
2013
39. Gilchrist J$, Das S$, Van Petegem F*, Bosmans F* (2013) Crystallographic insights into sodium-channel modulation by the β4 subunit. Proc Natl Acad Sci USA 110, E5016-5024
*Shared corresponding authorship..
38. Zhong X, Liu Y, Zhu L, Meng X, Wang R, Van Petegem F, Wagenknecht T, Chen SR, Liu Z (2013) Conformational Dynamics inside Amino-Terminal Disease Hotspot of Ryanodine Receptor. Structure 21, 2051-60.
37. Ellis SJ, Goult BT, Fairchild MJ, Harris NJ, Long J, Lobo PA, Czerniecky S, Van Petegem F, Schock F, Peifer M, Tanentzapf G (2013) Talin Autoinhibition Is Required for Morphogenesis. Current Biology 23, 1825-1833
36. Amador FJ, Kimlicka L, Stathopulos PB, Gasmi-Seabrook G, MacLennan DH, Van Petegem F*, Ikura M* (2013) Type 2 Ryanodine Receptor Domain A contains a unique and dynamic α-helix that transitions to a β-strand in a mutant linked with a heritable cardiomyopathy. J. Mol. Biol. 425, 4034-4046 *Shared corresponding authorship.
35. Kimlicka L, Tung C-C, Carlsson AC, Lobo PA, Yuchi Z, Van Petegem F (2013) The cardiac Ryanodine Receptor N-terminal region contains an anion binding site that is targeted by disease mutations. Structure 21, 1-10.
34. Liu Y, Kimlicka L, Hiess F, Tian X, Wang R, Zhang L, Jones PJ, Van Petegem F, Chen SR (2013) The CPVT-associated RyR2 mutation G230C enhances store overload-induced Ca2+ release and destabilizes the NH2-terminal domains. Biochem J. 454,123-131.
33. Kimlicka L, Lau K, Tung CC, Van Petegem F (2013) Disease mutations in the Ryanodine Receptor N-terminal region couple to a mobile intersubunit interface. Nature Comm. 4:1506.
2012
32. Van Petegem F*, Lobo PA, Ahern CA* (2012) Seeing the forest through the trees: towards a unified view on physiological calcium regulation of voltage-gated sodium channels. Biophys. J. 11,2243-2251 * Shared corresponding authorship
31. Van Petegem F (2012) Ryanodine Receptors: Structure and Function. J. Biol. Chem. 287, 31624-31632.
30. Yuchi M, Lau K, Van Petegem F (2012) Disease mutations in the Ryanodine Receptor Central Region: Crystal structures of a phosphorylation hot spot domain. Structure 20, 1201-1211.
29. Xiaohu L, Chen M, Lobo PA, An J, Cheng SWG, Moradian A, Morin G, Van Petegem F*, Jiang X* (2012) Molecular and Structural Characterization of the SH3 Domain of AHI-1 in Regulation of Cellular Resistance of BCR-ABL+ Chronic Myeloid Leukemia Cells to Tyrosine Kinase Inhibitors. Proteomics 12, 2094-2106. * Shared corresponding authorship
28. Sarhan M, Van Petegem F*, Ahern CA* (2012) Crystallographic basis for calcium regulation of sodium channels. Proc. Nat. Acad. Sci. USA 109, 3558-3563
* Co-supervised student and shared corresponding authorship
27. Chow SS, Van Petegem F, Accili EA (2012) Energetics of cyclic AMP binding to HCN c-terminus reveals negative cooperativity. J. Biol. Chem. 287, 600-606
2011
26. Kimlicka L, Van Petegem F (2011) The Structural Biology of Ryanodine Receptors. Sci China: Life Sciences 54, 712-724.
25. Lobo PA, Kimlicka L, Tung CC, Van Petegem F (2011) The RyR2 exon3 deletion is rescued by beta strand switching. Structure 19,790-798.
24. Yuchi Z, Van Petegem F (2011) Common allosteric mechanisms between ryanodine and inositol-1,4,5-trisphosphate receptors. Channels 5, 120-123.
2010
23. Kim EY, Rumpf CH, Van Petegem F, Arant R, Findeisen F, Cooley ES, Isacoff EY, Minor DL (2010) Multiple C-terminal tail Ca2+/CaMs regulate CaV1.2 function but do not mediate channel dimerization. EMBO J. 29, 3924-3938
22. Tung C-C, Lobo PA, Kimlicka L, Van Petegem F (2010) The ryanodine receptor N-terminal disease hot spot forms a cytoplasmic vestibule. Nature 468, 585-588.
21. Okamoto S, Van Petegem F, Patrauchan MA, Eltis LD (2010) AnhE, a metallochaperone involved in the maturation of a cobalt-dependent nitrile hydratase. J. Biol. Chem. 285, 25126-25133.
2009
20. Janssens ME, Geysen D, Broos K, De Goeyse I, Robben J, Van Petegem F, Timmermans J-P, Guisez Y (2009) Folding properties of the hepatitis B core as a carrier protein for vaccination research. Amino Acids 38, 1617-1626.
19. Sarhan MS, Van Petegem F*, Ahern CA* (2009) A double tyrosine motif in the cardiac sodium channel Domain III-IV linker couples calcium dependent calmodulin binding to inactivation gating. J. Biol. Chem. 284,33265-33274. * Co-supervised graduate student
18. Lobo PA, Van Petegem F (2009) Crystal structures of the N-terminal domains of cardiac and skeletal muscle ryanodine receptors: insights into disease mutations. Structure 17,1505-1514.
17. Cheung TYS, Fairchild M, Zarivach R, Tanentzapf G, Van Petegem F (2009) Crystal Structure of the Talin Integrin Binding Domain 2. J. Mol. Biol. 387, 787-793.
2008 and earlier
16. Kim EY, Rumpf CH, Fujiwara Y, Cooley ES, Van Petegem F, Minor DL (2008) Structures of CaV2 Ca2+/CaM-IQ domain complexes reveal binding modes that underlie calcium-dependent inactivation and facilitation. Structure 16, 1455-1467.
15. De Vos D, Xu Y, Aerts T, Van Petegem F, Van Beeumen J (2008) Crystal structure of Sulfolobus acidocaldarius aspartate carbamoyltransferase in complex with its allosteric activator CTP. Biochem Biophys Res Commun. 372, 40-44.
14. Van Petegem F, Duderstadt KE, Clark KA, Wang M, Minor DL (2008) Alanine-scanning mutagenesis defines a conserved energetic hotspot in the CaVα AID-CaVβ interaction site that is critical for channel modulation. Structure 16, 280-294.
13. Van Petegem F, De Vos D, Savvides S, Vergauwen B, Van Beeumen J (2007) Understanding nicotinamide dinucleotide cofactor and substrate specificity in class I flavoprotein disulfide oxidoreductases: Crystallographic analysis of a glutathione amide reductase. J. Mol. Biol. 374,883-889.
12. Van Petegem F, Minor DL (2006) The structural biology of voltage-gated calcium channel function and regulation. Biochem. Soc. Trans. 34,887-893.
11. Van Petegem F, Chatelain FC, Minor DL (2005) Insights into voltage-gated calcium channel regulation from the structure of the CaV1.2 IQ domain-Ca2+/calmodulin complex. Nature Struct. Mol. Biol. 12, 1108-1115.
10. Dumortier C, Fitch J, Van Petegem F, Vermeulen W, Meyer TE, Van Beeumen J, Cusanovich MA. (2004) Protein dynamics in the region of the sixth ligand methionine revealed by studies of imidazole binding to Rhodobacter capsulatus cytochrome c2 hinge mutants. Biochemistry. 43, 7717-7724.
9. De Vos D, Van Petegem F, Remaut H, Legrain C, Glansdorff N, Van Beeumen J (2004) Crystal structure of T state aspartate carbamoyltransferase of the hyperthermophilic archaeon Sulfolobus acidocaldarius. J. Mol. Biol. 339,887-900.)
8. Van Petegem F, Clark KA, Chatelain FC, Minor DL (2004) Structure of a complex between a voltage-gated calcium channel beta-subunit and an alpha-subunit domain. Nature 429, 671-675.
7. Aghajari N* & Van Petegem F*, Villeret V, Chessa JP, Gerday C, Haser R, and Van Beeumen J (2003) Crystal structures of a psychrophilic metalloprotease provide new insights into catalysis by cold-adapted proteases. Proteins: Struct. Funct. Genet. 50, 636-647.
*Both authors contributed equally to the work
6. Van Petegem F, Collins T, Meuwis MA, Gerday C, Feller G, and Van Beeumen J (2002) The structure of a cold-adapted family 8 xylanase at 1.3Å resolution, structural adaptations to cold and investigation of the active site. J. Biol. Chem. 278, 7531-7539.
5. Van Petegem F, Vandenberghe I, Bhat MK, and Van Beeumen J (2002) Atomic resolution structure of the major endoglucanase from Thermoascus aurantiacus. Biochem. Biophys. Res. Commun. 296, 161-166.
4. Van Petegem F, Collins T, Meuwis MA, Gerday C, Feller G, and Van Beeumen J (2002) Crystallization and preliminary X-ray analysis of a xylanase from the psychrophile Pseudoalteromonas haloplanktis. Acta Cryst. D58, 1494-1496.
3. Vergauwen B, Van Petegem F, Remaut H, Pauwels F, and Van Beeumen J (2002) Crystallization and preliminary X-ray crystallographic analysis of glutathione amide reductase from Chromatium gracile. Acta Cryst. D58, 339-340.
2. Van Petegem F, Contreras H, Contreras R, and Van Beeumen J (2001) Trichoderma reesei alpha-1,2-mannosidase: structural basis for the cleavage of four consecutive mannose residues. J. Mol. Biol. 312, 157-165.
1. Georlette D, Jonsson ZO, Van Petegem F, Chessa J, Van Beeumen J, Hubscher U, Gerday C (2000) A DNA ligase from the psychrophile Pseudoalteromonas haloplanktis gives insights into the adaptation of proteins to low temperatures. Eur. J. Biochem. 267, 3502-3512.